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Proceedings Paper

Resonance Raman imaging for detecting and monitoring molecular pathological changes in human brain tumors related to Warburg effect
Author(s): Yan Zhou; Cheng-hui Liu; Ke Zhu; Chunyuan Zhang; Yang Yang; Xinguang Yu; Hailong Hu; Gangge Cheng; Binlin Wu; Lingyan Shi; Robert R. Alfano
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Paper Abstract

The goal of the research is to determine the prognostic molecular pathological changes in components and composition, for human brain glioma gradings in comparison with normal tissues in three-dimensional Raman imaging profiles by visible Resonance Raman (VRR) imaging.

VRR images from twenty-five specimens including three healthy tissues, one normal control, and twenty-one glioma tissues of grades II, II-III and III-IV with histology examination were measured and investigated using WITec300R confocal micro Raman imaging system with laser excitation of 532nm.

Two-dimensional RR spectral mappings performed in 20μm x 20μm generated 400 images which integrated the intensity of the specific biochemical bonds as the third dimension. The three-dimension (3D) map demonstrated the spatial distributions of three selected sets of RR spectra of molecular biomarkers, and revealed significant differences in the spectra between normal and glioma tissues of different grades due to the composition changes in key molimageecules. These RR molecular spectral fingerprints have displayed: a clear enhancement of RR vibrational modes at 1129-1131cm-1 and 2934cm-1 which are supposed to be arising from lipoproteins; evident decreased RR vibrational modes at 1442cm-1 and 2854cm-1 which are from saturated fatty acids bonds in all-grades of glioma brain tissues compared with normal tissues; and the enhanced RR spectral modes of 1129 cm-1 and 2938cm-1 which suggest contribution from lactate. These findings may provide a novel proof for anaerobic glycolysis metabolic process in brain glioma cancer tissues that has been explained by Warburg effects.

Paper Details

Date Published: 21 February 2018
PDF: 6 pages
Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104891C (21 February 2018); doi: 10.1117/12.2290794
Show Author Affiliations
Yan Zhou, The General Hospital of the Air Force, PLA (China)
Cheng-hui Liu, The City College of New York (United States)
Ke Zhu, Institute of Physics (China)
Chunyuan Zhang, The City College of New York (United States)
Yang Yang, Institute of Physics (China)
Xinguang Yu, PLA General Hospital (China)
Hailong Hu, WITec GmbH (China)
Gangge Cheng, The General Hospital of the Air Force, PLA (China)
Binlin Wu, Southern Connecticut State Univ. (United States)
Lingyan Shi, Columbia Univ. (United States)
Robert R. Alfano, The City College of New York (United States)

Published in SPIE Proceedings Vol. 10489:
Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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