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Proceedings Paper

Simultaneous estimation of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy
Author(s): Izumi Nishidate; Wares MD. Abdul; Mizuki Ohtsu; Kazuya Nakano; Hideaki Haneishi
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Paper Abstract

We propose a method to estimate transcutaneous bilirubin, hemoglobin, and melanin based on the diffuse reflectance spectroscopy. In the proposed method, the Monte Carlo simulation-based multiple regression analysis for an absorbance spectrum in the visible wavelength region (460-590 nm) is used to specify the concentrations of bilirubin (Cbil), oxygenated hemoglobin (Coh), deoxygenated hemoglobin (Cdh), and melanin (Cm). Using the absorbance spectrum calculated from the measured diffuse reflectance spectrum as a response variable and the extinction coefficients of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, as predictor variables, multiple regression analysis provides regression coefficients. Concentrations of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, are then determined from the regression coefficients using conversion vectors that are numerically deduced in advance by the Monte Carlo simulations for light transport in skin. Total hemoglobin concentration (Cth) and tissue oxygen saturation (StO2) are simply calculated from the oxygenated hemoglobin and deoxygenated hemoglobin. In vivo animal experiments with bile duct ligation in rats demonstrated that the estimated Cbil is increased after ligation of bile duct and reaches to around 20 mg/dl at 72 h after the onset of the ligation, which corresponds to the reference value of Cbil measured by a commercially available transcutaneous bilirubin meter. We also performed in vivo experiments with rats while varying the fraction of inspired oxygen (FiO2). Coh and Cdh decreased and increased, respectively, as FiO2 decreased. Consequently, StO2 was dramatically decreased. The results in this study indicate potential of the method for simultaneous evaluation of multiple chromophores in skin tissue.

Paper Details

Date Published: 20 February 2018
PDF: 9 pages
Proc. SPIE 10501, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, 1050116 (20 February 2018); doi: 10.1117/12.2290559
Show Author Affiliations
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)
Wares MD. Abdul, Tokyo Univ. of Agriculture and Technology (Japan)
Mizuki Ohtsu, Tokyo Univ. of Agriculture and Technology (Japan)
Kazuya Nakano, Chiba Univ. (Japan)
Hideaki Haneishi, Chiba Univ. (Japan)


Published in SPIE Proceedings Vol. 10501:
Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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