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In vivo, noncontact, real-time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model
Author(s): Seth Fillioe; Kyle Kelly Bishop; Alexander Vincent Struck Jannini; Jon Kim; Ricky McDonough; Steve Ortiz; Jerry Goodisman; Julie Hasenwinkel; J. Chaiken
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Paper Abstract

We report a small study to test a methodology for real-time probing of chemical and physical changes in spinal cords in the immediate aftermath of a localized contusive injury. Raman spectroscopy, optical profilimetry and scanning NIR autofluorescence images were obtained simultaneously in vivo, within a 3 x 7 mm field, on spinal cords that had been surgically exposed between T9 and T10. The collected data was used alone and/or combined in a unique algorithm. A total of six rats were studied in two N=3 groups i.e. Injured and Control. A single 830 nm laser (100 μm round spot) was either 1) spatially scanned across the cord or 2) held at a specified location relative to the injury for a longer period of time to improve signal to noise in the Raman spectra. Line scans reveal photobleaching effects and surface profiles possibly allowing identification of the anterior median longitudinal artery. Analysis of the Raman spectra suggest that the tissues were equally hypoxic for both the control and injured animals i.e. a possible artifact of anesthesia and surgery. On the other hand, only injured cords display Raman features possibly indicating that extensive, localized protein phosphorylation occurs in minutes following spinal cord trauma.

Paper Details

Date Published: 19 February 2018
PDF: 14 pages
Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104890B (19 February 2018); doi: 10.1117/12.2290500
Show Author Affiliations
Seth Fillioe, Syracuse Univ. (United States)
Kyle Kelly Bishop, Syracuse Univ. (United States)
Alexander Vincent Struck Jannini, Syracuse Univ. (United States)
Jon Kim, Syracuse Univ. (United States)
Ricky McDonough, Syracuse Univ. (United States)
Steve Ortiz, Syracuse Univ. (United States)
Jerry Goodisman, Syracuse Univ. (United States)
Julie Hasenwinkel, Syracuse Univ. (United States)
J. Chaiken, Syracuse Univ. (United States)

Published in SPIE Proceedings Vol. 10489:
Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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