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Proceedings Paper

In-vivo fluorescence detection of breast cancer growth factor receptors by fiber-optic probe
Author(s): Gilbert Bustamante; Bingzhi Wang; Frank DeLuna; LuZhe Sun; Jing Yong Ye
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Paper Abstract

Breast cancer treatment options often include medications that target the overexpression of growth factor receptors, such as the proto-oncogene human epidermal growth factor receptor 2 (HER2/neu) and epidermal growth factor receptor (EGFR) to suppress the abnormal growth of cancerous cells and induce cancer regression. Although effective, certain treatments are toxic to vital organs, and demand assurance that the pursued receptor is present at the tumor before administration of the drug. This requires diagnostic tools to provide tumor molecular signatures, as well as locational information. In this study, we utilized a fiber-optic probe to characterize in vivo HER2 and EGFR overexpressed tumors through the fluorescence of targeted dyes. HER2 and EGFR antibodies were conjugated with ICG-Sulfo-OSu and Alexa Fluor 680, respectively, to tag BT474 (HER2+) and MDA-MB-468 (EGFR+) tumors. The fiber was inserted into the samples via a 30-gauge needle. Different wavelengths of a supercontinuum laser were selected to couple into the fiber and excite the corresponding fluorophores in the samples. The fluorescence from the dyes was collected through the same fiber and quantified by a time-correlated single photon counter. Fluorescence at different antibody-dye concentrations was measured for calibration. Mice with subcutaneous HER2+ and/or EGFR+ tumors received intravenous injections of the conjugates and were later probed at the tumor sites. The measured fluorescence was used to distinguish between tumor types and to calculate the concentration of the antibody-dye conjugates, which were detectable at levels as low as 40 nM. The fiber-optic probe presents a minimally invasive instrument to characterize the molecular signatures of breast cancer in vivo.

Paper Details

Date Published: 19 February 2018
PDF: 11 pages
Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104890E (19 February 2018); doi: 10.1117/12.2290464
Show Author Affiliations
Gilbert Bustamante, The Univ. of Texas at San Antonio (United States)
Bingzhi Wang, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Frank DeLuna, The Univ. of Texas at San Antonio (United States)
LuZhe Sun, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Jing Yong Ye, The Univ. of Texas at San Antonio (United States)


Published in SPIE Proceedings Vol. 10489:
Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos, Editor(s)

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