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Proceedings Paper

Identification of hydrodynamic forces around 3D surrogates using particle image velocimetry in a microfluidic channel
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Paper Abstract

Previous studies have demonstrated that flow-induced shear stress induces a motile and aggressive tumor phenotype in a microfluidic model of 3D ovarian cancer. However, the magnitude and distribution of the hydrodynamic forces that influence this biological modulation on the 3D cancer nodules are not known. We have developed a series of numerical and experimental tools to identify these forces within a 3D microchannel. In this work, we used particle image velocimetry (PIV) to find the velocity profile using fluorescent micro-spheres as surrogates and nano-particles as tracers, from which hydrodynamic forces can be derived. The fluid velocity is obtained by imaging the trajectory of a range of florescence nano-particles (500–800 μm) via confocal microscopy. Imaging was done at different horizontal planes and with a 50 μm bead as the surrogate. For an inlet current rate of 2 μl/s, the maximum velocity at the center of the channel was 51 μm/s. The velocity profile around the sphere was symmetric which is expected since the flow is dominated by viscous forces as opposed to inertial forces. The confocal PIV was successfully employed in finding the velocity profile in a microchannel with a nodule surrogate; therefore, it seems feasible to use PIV to investigate the hydrodynamic forces around 3D biological models.

Paper Details

Date Published: 19 February 2018
PDF: 8 pages
Proc. SPIE 10491, Microfluidics, BioMEMS, and Medical Microsystems XVI, 1049103 (19 February 2018); doi: 10.1117/12.2290372
Show Author Affiliations
Sepideh Afshar, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Shubhankar Nath, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Utkan Demirci, Stanford Univ. (United States)
Tayyaba Hasan, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Giuliano Scarcelli, Univ. of Maryland, College Park (United States)
Imran Rizvi, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Walfre Franco, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 10491:
Microfluidics, BioMEMS, and Medical Microsystems XVI
Bonnie L. Gray; Holger Becker, Editor(s)

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