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Proceedings Paper

Characterization of a reflective objective with multiphoton microscopy
Author(s): Mohammad M. Kabir; Aakash M. Choubal; Mayandi Sivaguru; Kimani C. Toussaint
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Paper Abstract

Reflective objectives (ROs) can reduce chromatic aberration across a wide wavelength range in multiphoton microscopy (MPM). However, a systematic characterization of the performance of ROs has not been carried out. In this paper, we analyze the performance of a 0.5 numerical-aperture (NA) RO and compare it with a 0.55 NA standard glass objective (SO), using two-photon fluorescence (TPF) and second-harmonic generation (SHG). For experiments extending ~1 octave in visible and NIR wavelengths, the SO introduces defocusing errors of ~25% for TPF images of sub-diffraction fluorescent beads and ~10% for SHG images of collagen fibers. For both imaging systems, the RO provides a corresponding error of ~4%. This work highlights the potential usefulness of ROs for multimodal MPM applications.

Paper Details

Date Published: 23 February 2018
PDF: 5 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982Z (23 February 2018); doi: 10.1117/12.2290305
Show Author Affiliations
Mohammad M. Kabir, Univ. of Illinois at Urbana-Champaign (United States)
Aakash M. Choubal, Univ. of Illinois at Urbana-Champaign (United States)
Mayandi Sivaguru, Univ. of Illinois at Urbana-Champaign (United States)
Kimani C. Toussaint, Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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