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Proceedings Paper

Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry
Author(s): Rami Shnaiderman; Georg Wissmeyer; Markus Seeger; Hector Estrada; Vasilis Ntziachristos
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Paper Abstract

Optoacoustic microscopy (OAM) has enabled high-resolution, label-free imaging of tissues at depths not achievable with purely optical microscopy. However, widespread implementation of OAM into existing epi-illumination microscopy setups is often constrained by the performance and size of the commonly used piezoelectric ultrasound detectors. In this work, we introduce a novel acoustic detector based on a π-phase-shifted fiber Bragg grating (π-FBG) interferometer embedded inside an ellipsoidal acoustic cavity. The cavity enables seamless integration of epi-illumination OAM into existing microscopy setups by decoupling the acoustic and optical paths between the microscope objective and the sample. The cavity also acts as an acoustic condenser, boosting the sensitivity of the π-FBG and enabling cost effective CW-laser interrogation technique. We characterize the sensor’s sensitivity and bandwidth and demonstrate hybrid OAM and second-harmonic imaging of phantoms and mouse tissue in vivo.

Paper Details

Date Published: 19 February 2018
PDF: 8 pages
Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942M (19 February 2018); doi: 10.1117/12.2290281
Show Author Affiliations
Rami Shnaiderman, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)
Georg Wissmeyer, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)
Markus Seeger, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)
Hector Estrada, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)
Vasilis Ntziachristos, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)


Published in SPIE Proceedings Vol. 10494:
Photons Plus Ultrasound: Imaging and Sensing 2018
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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