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Mitochondrial protein knockouts induce changes in the fluorescence lifetimes of NAD(P)H and FAD (Conference Presentation)
Author(s): Amani Gillette; Peter Favreau; Jarred Rensvold; Ava VanDommelen; David Pagliarini; Melissa Skala

Paper Abstract

Current technologies for cellular metabolomics are complex, costly, and lack single-cell resolution. We propose optical metabolic imaging (OMI) as a tool to noninvasively monitor cellular metabolism on a single-cell level. OMI uses two-photon microscopy and time correlated single photon counting to measure the fluorescence lifetime and intensity of endogenous metabolic coenzymes NAD(P)H and FAD. However, the biochemical basis of changes in OMI parameters such as redox ratio (NAD(P)H intensity divided by FAD intensity), NAD(P)H lifetime, and FAD lifetime remains unclear. A thorough understanding of these biochemical sources of contrast is needed to interpret OMI studies of drug efficacy, and thus leverage OMI as a tool for drug screening and metabolic research. To address this issue, we profiled 11 CRISPR-mediated knockout human cell lines with single gene deletions corresponding to mitochondrial proteins involved in characterized pathways, and complexes ranging from oxidative phosphorylation to mitochondrial structure. The wild-type and knockout cells were plated at an equal density on 35mm imaging dishes in parallel with additional plates for other analyses. After 48hrs, cells were imaged or collected for mass spectrometry-based metabolomic, proteomic, and lipidomic analyses. OMI of the wild-type and knockout haploid cells yielded statistically significant differences in redox ratio, NAD(P)H and FAD mean lifetimes (ANOVA, p<0.005) that were specific to the knockout. Comparisons between OMI and parallel multi-omic analysis of each knockout are currently underway. This comprehensive dataset will provide a better understanding of the biochemical basis for OMI measurements, which could enable robust, single-cell monitoring of metabolic activity.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104980F (14 March 2018); doi: 10.1117/12.2290247
Show Author Affiliations
Amani Gillette, Univ. of Wisconsin-Madison (United States)
Morgridge Institute for Research (United States)
Peter Favreau, Morgridge Institute for Research (United States)
Jarred Rensvold, Morgridge Institute for Research (United States)
Ava VanDommelen, Morgridge Institute for Research (United States)
David Pagliarini, Morgridge Institute for Research (United States)
Univ. of Wisconsin-Madison (United States)
Melissa Skala, Morgridge Institute for Research (United States)
Univ. of Wisconsin-Madison (United States)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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