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Proceedings Paper

Imaging the developing heart: synchronized time-lapse microscopy during developmental changes
Author(s): Carl J. Nelson; Charlotte Buckley; John J. Mullins; Martin A. Denvir; Jonathan Taylor
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Paper Abstract

How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991F (23 February 2018); doi: 10.1117/12.2290191
Show Author Affiliations
Carl J. Nelson, Univ. of Glasgow (United Kingdom)
Charlotte Buckley, The Univ. of Edinburgh (United Kingdom)
John J. Mullins, The Univ. of Edinburgh (United Kingdom)
Martin A. Denvir, The Univ. of Edinburgh (United Kingdom)
Jonathan Taylor, Univ. of Glasgow (United Kingdom)


Published in SPIE Proceedings Vol. 10499:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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