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Large scale serial two-photon microscopy to investigate local vascular changes in whole rodent brain models of Alzheimer's disease
Author(s): P. Delafontaine-Martel; J. Lefebvre; R. Damseh; A. Castonguay; P. Tardif; F. Lesage
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Paper Abstract

In this study, an automated serial two-photon microscope was used to image a fluorescent gelatin filled rodent’s brain in 3D. A method to compute vascular density using automatic segmentation was combined with coregistration techniques to build group-level vasculature metrics. By studying the medial prefrontal cortex and the hippocampal formation of 3 age groups (2, 4.5 and 8 months old), we compared vascular density for both WT and an Alzheimer model transgenic brain (APP/PS1). We observe a loss of vascular density caused by the ageing process and we propose further analysis to confirm our results.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982O (23 February 2018); doi: 10.1117/12.2290060
Show Author Affiliations
P. Delafontaine-Martel, Ecole Polytechnique de Montréal (Canada)
J. Lefebvre, Ecole Polytechnique de Montréal (Canada)
R. Damseh, Ecole Polytechnique de Montréal (Canada)
A. Castonguay, Ecole Polytechnique de Montréal (Canada)
P. Tardif, Ecole Polytechnique de Montréal (Canada)
F. Lesage, Ecole Polytechnique de Montréal (Canada)
Montréal Health Institute (Canada)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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