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Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy
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Paper Abstract

The Analog mean delay (AMD) method is a multiphoton detection fluorescence lifetime measurement method. We have investigated the effect of the linearity of a photodetector response on the performance of the AMD method, which is a multiphoton detected fluorescence lifetime measurement method. A Monte-Carlo simulation scheme was adapted to generate various electronic signals for the AMD method with different linearity conditions of a photodetector. It is found that the photon economy is better if a photodetector with better linearity is used especially when the number of detected photons per pulse is low.

Paper Details

Date Published: 23 February 2018
PDF: 4 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982G (23 February 2018); doi: 10.1117/12.2290059
Show Author Affiliations
Dongeun Kim, Yonsei Univ. (Korea, Republic of)
Wonsang Hwang, Yonsei Univ. (Korea, Republic of)
Youngjae Won, Yonsei Univ. (Korea, Republic of)
Sucbei Moon, Yonsei Univ. (Korea, Republic of)
Dug Young Kim, Yonsei Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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