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Proceedings Paper

Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens
Author(s): M. Quintavalla; P. Pozzi; Michelle Verhaegen; Hielke Bijlsma; Hans Verstraete; S. Bonora
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Paper Abstract

Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.

Paper Details

Date Published: 23 February 2018
PDF: 5 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981X (23 February 2018); doi: 10.1117/12.2290036
Show Author Affiliations
M. Quintavalla, CNR-Institute for Photonics and Nanotechnology (Italy)
P. Pozzi, TU Delft (Netherlands)
Michelle Verhaegen, TU Delft (Netherlands)
Hielke Bijlsma, TU Delft (Netherlands)
Hans Verstraete, TU Delft (Netherlands)
S. Bonora, CNR-Institute for Photonics and Nanotechnology (Italy)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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