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Photoacoustic microscopy of single cells employing an intensity-modulated diode laser
Author(s): Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Manoj Kumar Dasa; Thomas A. Klar; Thomas Berer
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Paper Abstract

In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-µm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.

Paper Details

Date Published: 19 February 2018
PDF: 7 pages
Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942L (19 February 2018); doi: 10.1117/12.2289978
Show Author Affiliations
Gregor Langer, Research Ctr. for Non Destructive Testing GmbH (Austria)
Bianca Buchegger, Johannes Kepler Univ. Linz (Austria)
Jaroslaw Jacak, Johannes Kepler Univ. Linz (Austria)
Manoj Kumar Dasa, TU Denmark (Denmark)
Thomas A. Klar, Johannes Kepler Univ. Linz (Austria)
Thomas Berer, Research Ctr. for Non Destructive Testing GmbH (Austria)

Published in SPIE Proceedings Vol. 10494:
Photons Plus Ultrasound: Imaging and Sensing 2018
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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