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Proceedings Paper

Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system
Author(s): Rou-Xuan Huang; Ying Fu; Wang Liu; Yu-Ting Ma; Bao-Yu Hsieh; Shu-Ching Chen; Mingjian Sun; Pai-Chi Li
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Paper Abstract

Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3 . To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.

Paper Details

Date Published: 19 February 2018
PDF: 8 pages
Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 1049462 (19 February 2018); doi: 10.1117/12.2289975
Show Author Affiliations
Rou-Xuan Huang, National Taiwan Univ. (Taiwan)
Ying Fu, Harbin Institute of Technology (China)
Wang Liu, Harbin Institute of Technology (China)
Yu-Ting Ma, National Taiwan Univ. Hospital (Taiwan)
Bao-Yu Hsieh, China Medical Univ. (Taiwan)
Shu-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
Mingjian Sun, Harbin Institute of Technology (China)
Pai-Chi Li, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10494:
Photons Plus Ultrasound: Imaging and Sensing 2018
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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