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Label-free cell viability assay using lens-free microscopy
Author(s): C. Allier; T. Bordy; L. Hervé; O. Cioni; G. Esteban; M. Pisaneschi; S. Morales
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Paper Abstract

We introduce a label-free technique based on lens-free microscopy to perform cell counting and cell viability assay. Without the use of any labelling, the discrimination between alive and dead cells is obtained by analyzing the cells on the basis of their holographic signature. We have assessed this novel technique by comparing the obtained results in terms of viability and cell counting against automatic optical counting and trypan blue staining as reference methods. The lensfree measurements agree very well with the reference techniques up to ~30.106 cells/ml. We found a coefficient of determination R2 of 0.99 and a slope of 1.01 for the viability measurements (N=84 CHO cell samples).

Paper Details

Date Published: 20 February 2018
PDF: 7 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970B (20 February 2018); doi: 10.1117/12.2289811
Show Author Affiliations
C. Allier, Univ. Grenoble Alpes, CEA-LETI (France)
T. Bordy, Univ. Grenoble Alpes, CEA-LETI (France)
L. Hervé, Univ. Grenoble Alpes, CEA-LETI (France)
O. Cioni, Univ. Grenoble Alpes, CEA-LETI (France)
G. Esteban, IPRASENSE SAS (France)
M. Pisaneschi, IPRASENSE SAS (France)
S. Morales, Univ. Grenoble Alpes, CEA-LETI (France)

Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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