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Laser induced forward transfer bioprinting of immune cells and chemoattractant proteins for immunological responses studies (Conference Presentation)
Author(s): Sara Lauzurica; Andrés Marquez; Carlos Molpeceres; Miguel Gómez-Fontela; Laura Notario; Pilar Lauzurica
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Paper Abstract

Laser bioprinting is a powerful tool in many biological fields due to its versatility in placing and construct different geometries of biological materials. The high accuracy and non-destructive nature of this method can be applied to the study of complex biological systems. In particular, single cell laser bioprinting helps to understand the relationships between cells and their local environment. Immunology is a transversal field that is governed by a complex network of genetic and signalling pathways subtending a network of interacting cells. In this context, mobility of the cells in a network along with their situation and the gene products they interact with, plays an important roll in the behaviour of the immune system. In this work we use a laser induced forward transfer blister assisted (BALIFT) approach to assess these cell-cell interaction and mobility in vitro. This method helps to understand properly the role of a cell in such networks to increase our knowledge of the immune system response. This work presents BALIFT bioprinting of single hematopoietic cells and chemoattractant proteins with high spatial resolution. In particular NK cells (natural killer), T-lymphocyte and chemokines and cytokine molecules are printed in specific patterns to study cell-cell and cell-environment interaction and cell migration. Whereby placing cellular components on a matrix previously designed on demand allow us to test the molecular interactions between lymphocytes and pathogens; as well as the generation of two-dimensional structures printed ad hoc in order to study the mechanisms of mobilization of immune system cells.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10523, Laser 3D Manufacturing V, 105230Q (14 March 2018); doi: 10.1117/12.2289782
Show Author Affiliations
Sara Lauzurica, Univ. Politécnica de Madrid (Spain)
Andrés Marquez, Univ. Politécnica de Madrid (Spain)
Carlos Molpeceres, Univ. Politécnica de Madrid (Spain)
Miguel Gómez-Fontela, Instituto de Salud Carlos III (Spain)
Laura Notario, Instituto de Salud Carlos III (Spain)
Pilar Lauzurica, Instituto de Salud Carlos III (Spain)

Published in SPIE Proceedings Vol. 10523:
Laser 3D Manufacturing V
Henry Helvajian; Bo Gu; Alberto Piqué, Editor(s)

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