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Proceedings Paper

Speckle-free and halo-free low coherent Mach-Zehnder quantitative-phase-imaging module as a replacement of objective lens in conventional inverted microscopes
Author(s): Toyohiko Yamauchi; Hidenao Yamada; Hisayuki Matsui; Osamu Yasuhiko; Yukio Ueda
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Paper Abstract

We developed a compact Mach-Zehnder interferometer module to be used as a replacement of the objective lens in a conventional inverted microscope (Nikon, TS100-F) in order to make them quantitative phase microscopes. The module has a 90-degree-flipped U-shape; the dimensions of the module are 160 mm by 120 mm by 40 mm and the weight is 380 grams. The Mach-Zehnder interferometer equipped with the separate reference and sample arms was implemented in this U-shaped housing and the path-length difference between the two arms was manually adjustable. The sample under test was put on the stage of the microscope and a sample light went through it. Both arms had identical achromatic lenses for image formation and the lateral positions of them were also manually adjustable. Therefore, temporally and spatially low coherent illumination was applicable because the users were able to balance precisely the path length of the two arms and to overlap the two wavefronts. In the experiment, spectrally filtered LED light for illumination (wavelength = 633 nm and bandwidth = 3 nm) was input to the interferometer module via a 50 micrometer core optical fiber. We have successfully captured full-field interference images by a camera put on the trinocular tube of the microscope and constructed quantitative phase images of the cultured cells by means of the quarter-wavelength phase shifting algorithm. The resultant quantitative phase images were speckle-free and halo-free due to spectrally and spatially low coherent illumination.

Paper Details

Date Published: 23 February 2018
PDF: 6 pages
Proc. SPIE 10503, Quantitative Phase Imaging IV, 105031V (23 February 2018); doi: 10.1117/12.2289529
Show Author Affiliations
Toyohiko Yamauchi, Hamamatsu Photonics K.K. (Japan)
Hidenao Yamada, Hamamatsu Photonics K.K. (Japan)
Hisayuki Matsui, Hamamatsu Photonics K.K. (Japan)
Osamu Yasuhiko, Hamamatsu Photonics K.K. (Japan)
Yukio Ueda, Hamamatsu Photonics K.K. (Japan)


Published in SPIE Proceedings Vol. 10503:
Quantitative Phase Imaging IV
Gabriel Popescu; YongKeun Park, Editor(s)

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