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Interferometric scattering (iSCAT) microscopy: studies of biological membrane dynamics
Author(s): Francesco Reina; Silvia Galiani; Dilip Shrestha; Erdinc Sezgin; B. Christoffer Lagerholm; Daniel Cole; Philipp Kukura; Christian Eggeling
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Paper Abstract

The study of the organization and dynamics of molecules in model and cellular membranes is an important topic in contemporary biophysics. Imaging and single particle tracking in this particular field, however, proves particularly demanding, as it requires simultaneously high spatio-temporal resolution and high signal-to-noise ratios. A remedy to this challenge might be Interferometric Scattering (iSCAT) microscopy, due to its fast sampling rates, label-free imaging capabilities and, most importantly, tuneable signal level output. Here we report our recent advances in the imaging and molecular tracking on phase-separated model membrane systems and live-cell membranes using this technique.

Paper Details

Date Published: 20 February 2018
PDF: 8 pages
Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000L (20 February 2018); doi: 10.1117/12.2289501
Show Author Affiliations
Francesco Reina, Univ. of Oxford (United Kingdom)
Silvia Galiani, Univ. of Oxford (United Kingdom)
Dilip Shrestha, Univ. of Oxford (United Kingdom)
Erdinc Sezgin, Univ. of Oxford (United Kingdom)
B. Christoffer Lagerholm, Univ. of Oxford (United Kingdom)
Daniel Cole, Univ. of Oxford (United Kingdom)
Philipp Kukura, Univ. of Oxford (United Kingdom)
Christian Eggeling, Univ. of Oxford (United Kingdom)
Friedrich-Schiller Univ. (Germany)
Leibniz Institute of Photonic Technology (Germany)


Published in SPIE Proceedings Vol. 10500:
Single Molecule Spectroscopy and Superresolution Imaging XI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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