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Proceedings Paper

Endoscopic tissue fluorescence life-time imaging by frequency doamin light-induced fluorescence
Author(s): Jerome C. Mizeret; Georges A. Wagnieres; A. Studzinski; C. Shangguan; Hubert van den Bergh
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Paper Abstract

An instrumentation is being developed to draw a fluorescence life-time map of tissue endoscopically. This fluorescence life-time of an endogenous or exogenous fluorochrome gives information about the physico-chemical environment which is thought to vary between normal and diseased tissue. The excitation light from a cw laser is modulated in amplitude at high frequencies by an electro-optic modulator and delivered to the endoscopic site through an optical fiber. The image of the tissue is spectrally split in two parts, the one being the backscattered excitation light, the other the fluorescence of the fluorochromes. Each image is focused on the photocathode of an image intensifier whose gain is modulated at the same frequency. By acquiring frames at different phases between the excitation and the emission, it is possible to calculate pixel by pixel the apparent fluorescence life-time of the corresponding tissue region.

Paper Details

Date Published: 27 December 1995
PDF: 9 pages
Proc. SPIE 2627, Optical Biopsies, (27 December 1995); doi: 10.1117/12.228902
Show Author Affiliations
Jerome C. Mizeret, Ecole Polytechnique Federale de Lausanne (Switzerland)
Georges A. Wagnieres, Ecole Polytechnique Federale de Lausanne (Switzerland)
A. Studzinski, Ecole Polytechnique Federale de Lausanne (Switzerland)
C. Shangguan, Ecole Polytechnique Federale de Lausanne (Switzerland)
Hubert van den Bergh, Ecole Polytechnique Federale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 2627:
Optical Biopsies
Rinaldo Cubeddu; Serge R. Mordon; Katarina Svanberg, Editor(s)

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