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Spectroscopic mapping with coherent IR and Raman microscopy (Conference Presentation)

Paper Abstract

In coherent Raman scattering microscopy, the sample is examined through the Raman-active modes. Although many molecular modes display a nonzero Raman cross-section, some modes are intrinsically weak and are better viewed through their dipole-allowed (IR-active) transition. A more complete spectroscopic assessment of the sample would thus include a mechanism to prove both Raman and IR-active modes. In this contribution, we combine coherent Raman microscopy with second- and third-order sum-frequency generation microscopy, providing sensitivity to all optically accessible vibrational modes. All techniques can be carried out on the same laser-scanning imaging platform, generating images at fast acquisition rates and with high spatial resolution. We show examples where the combination of these techniques reveal new information about the tissue.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981G (14 March 2018); doi: 10.1117/12.2288589
Show Author Affiliations
Eric O. Potma, Univ. of California, Irvine (United States)
Richard Prince, Univ of California Irvine (United States)
Adam M. Hanninen, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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