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A portable measuring system for a competitive binding glucose biosensor
Author(s): Lydia E. Colvin; A. Kristen Means; Melissa A. Grunlan; Gerard L. Coté
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Paper Abstract

Central to minimizing the long- and short-term complications associated with diabetes is careful monitoring and maintenance of blood glucose at normal levels. Towards replacing conventionally used finger-prick glucose testing, indwelling continuous glucose monitors (CGMs) based on amperometric electrodes have been introduced to the market. Envisioned to lead to a CGM with an increased lifetime, we report herein a fluorescently-labeled competitive binding assay contained within a hydrogel membrane whose glucose response is measured via a novel portable system. The optical system design included a laser source, bifurcated fiber, laser filter and simple fiber coupled spectrometer to obtain the change in FRET pair ratio of the assay. Glucose response of the assay in free solution was measured using this system across the physiologic range (0-200 mg/dL). The FRET pair ratio signal was seen to increase with glucose and the standard error of calibration was 22.42 mg/dL with a MARD value of 14.85%. When the assay was contained within the hydrogel membrane’s central cavity and similarly analyzed, the standard error increased but the assay maintained its reversibility.

Paper Details

Date Published: 20 February 2018
PDF: 7 pages
Proc. SPIE 10501, Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, 1050107 (20 February 2018); doi: 10.1117/12.2288531
Show Author Affiliations
Lydia E. Colvin, Texas A&M Univ. (United States)
A. Kristen Means, Texas A&M Univ. (United States)
Melissa A. Grunlan, Texas A&M Univ. (United States)
Gerard L. Coté, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 10501:
Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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