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Proceedings Paper

Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy
Author(s): Paolo Pozzi; Dean Wilding; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen
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Paper Abstract

In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance cmos camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.

Paper Details

Date Published: 22 February 2018
PDF: 5 pages
Proc. SPIE 10546, Emerging Digital Micromirror Device Based Systems and Applications X, 105460D (22 February 2018); doi: 10.1117/12.2288436
Show Author Affiliations
Paolo Pozzi, Technische Univ. Delft (Netherlands)
Dean Wilding, Technische Univ. Delft (Netherlands)
Oleg Soloviev, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
ITMO Univ. (Russian Federation)
Gleb Vdovin, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
ITMO Univ. (Russian Federation)
Michel Verhaegen, Technische Univ. Delft (Netherlands)


Published in SPIE Proceedings Vol. 10546:
Emerging Digital Micromirror Device Based Systems and Applications X
Michael R. Douglass; Benjamin L. Lee, Editor(s)

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