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Profiling pleural effusion cells by a diffraction imaging method
Author(s): Safaa Al-Qaysi; Heng Hong; Yuhua Wen; Jun Q. Lu; Yuanming Feng; Xin-Hua Hu
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Paper Abstract

Assay of cells in pleural effusion (PE) is an important means of disease diagnosis. Conventional cytology of effusion samples, however, has low sensitivity and depends heavily on the expertise of cytopathologists. We applied a polarization diffraction imaging flow cytometry method on effusion cells to investigate their features. Diffraction imaging of the PE cell samples has been performed on 6000 to 12000 cells for each effusion cell sample of three patients. After prescreening to remove images by cellular debris and aggregated non-cellular particles, the image textures were extracted with a gray level co-occurrence matrix (GLCM) algorithm. The distribution of the imaged cells in the GLCM parameters space was analyzed by a Gaussian Mixture Model (GMM) to determine the number of clusters among the effusion cells. These results yield insight on textural features of diffraction images and related cellular morphology in effusion samples and can be used toward the development of a label-free method for effusion cells assay.

Paper Details

Date Published: 20 February 2018
PDF: 7 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971J (20 February 2018); doi: 10.1117/12.2288386
Show Author Affiliations
Safaa Al-Qaysi, East Carolina Univ. (United States)
Al-Mustansiriya Univ. (Iraq)
Heng Hong, East Carolina Univ. (United States)
Yuhua Wen, Hunan Institute of Science and Technology (China)
Jun Q. Lu, East Carolina Univ. (United States)
Hunan Institute of Science and Technology (China)
Yuanming Feng, East Carolina Univ. (United States)
Xin-Hua Hu, East Carolina Univ. (United States)
Hunan Institute of Science and Technology (China)


Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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