Share Email Print
cover

Proceedings Paper

Brillouin scattering imaging of stiffness heterogeneity in multicellular systems (Conference Presentation)
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Brillouin scattering microscopy is the only potential tool to realize microscopic mapping of mechanical property in multicellular system (i.e. colony, tissue) with sub-cell resolution. We built a laser-scanning Brillouin microscope system for our biological study on spatial heterogeneity of stiffness in multicellular system. High-numerical-aperture (NA) objective lens (NA ≥ 0.7) and a dual-VIPA based spectrometer were employed to achieve high spatial resolution and high sensitivity, respectively. Addition of a spatial filter at a Fourier plane of the EMCCD detector surface effectively rejected strongly reflected excitation light without loss of Brillouin scattering signal, which accordingly allowed us to observe cells just above glass substrate surface. We performed three-dimensional imaging of Brillouin scattering to see three-dimensional stiffness distribution within a multicellular system. It was found that cells in relatively central region or in close vicinity of glass substrate have higher stiffness, which agrees to biological prediction. We also found presence of anomaly cells with much higher elasticity than surrounding cells. The stiffness imaging was applied to various kinds of multicellular systems including ES cell colonies upon differentiation and artificial tissue grown from iPS cells. The results certified the effectiveness of Brillouin scattering microscope in mechanobiology, developmental biology and regenerative medicine. Several practical issues for biomedical application will also be discussed.

Paper Details

Date Published: 15 March 2018
PDF
Proc. SPIE 10496, Optical Elastography and Tissue Biomechanics V, 104960R (15 March 2018); doi: 10.1117/12.2288381
Show Author Affiliations
Taro Ichimura, RIKEN Quantitative Biology Ctr. (Japan)
Tomonobu M. Watanabe, RIKEN Quantitative Biology Ctr. (Japan)


Published in SPIE Proceedings Vol. 10496:
Optical Elastography and Tissue Biomechanics V
Kirill V. Larin; David D. Sampson, Editor(s)

© SPIE. Terms of Use
Back to Top