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Development towards compact nitrocellulose interferometric biochips for dry eye diagnosis based on MMP9, S100A6 and CST4 biomarkers using a Point-of-Care device
Author(s): Beatriz Santamaría; María Fe Laguna; David López-Romero; A. López-Hernandez; F. J. Sanza; A. Lavín; R. Casquel; M. Maigler; M. Holgado
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Paper Abstract

A novel compact optical biochip based on a thin layer-sensing BICELL surface of nitrocellulose is used for in-situ labelfree detection of dry eye disease (DED). In this work the development of a compact biosensor that allows obtaining quantitative diagnosis with a limited volume of sample is reported. The designed sensors can be analyzed with an optical integrated Point-of-Care read-out system based on the “Increase Relative Optical Power” principle which enhances the performance and Limit of Detection. Several proteins involved with dry eye dysfunction have been validated as biomarkers. Presented biochip analyzes three of those biomarkers: MMP9, S100A6 and CST4. BICELLs based on nitrocellulose permit to immobilize antibodies for each biomarker recognition. The optical response obtained from the biosensor through the readout platform is capable to recognize specifically the desired proteins in the concentrations range for control eye (CE) and dry eye syndrome (DES). Preliminary results obtained will allow the development of a dry eye detection device useful in the area of ophthalmology and applicable to other possible diseases related to the eye dysfunction.

Paper Details

Date Published: 19 February 2018
PDF: 7 pages
Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 1047424 (19 February 2018); doi: 10.1117/12.2288257
Show Author Affiliations
Beatriz Santamaría, Univ. Politécnica de Madrid (Spain)
María Fe Laguna, Univ. Politécnica de Madrid (Spain)
David López-Romero, BioOptical Detection, Ctr. de Empresas (Spain)
A. López-Hernandez, Univ. Politécnica de Madrid (Spain)
F. J. Sanza, BioOptical Detection, Ctr. de Empresas (Spain)
A. Lavín, Technical Univ. of Madrid (Spain)
R. Casquel, Univ. Politécnica de Madrid (Spain)
M. Maigler, BioOptical Detection, Ctr. de Empresas (Spain)
M. Holgado, Univ. Politécnica de Madrid (Spain)


Published in SPIE Proceedings Vol. 10474:
Ophthalmic Technologies XXVIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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