Share Email Print

Proceedings Paper • new

Cell-based quantification of biomarkers from an ultra-fast microfluidic immunofluorescent staining: application to human breast cancer cell lines
Author(s): D. Migliozzi; H. T. Nguyen; M. A. M. Gijs
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Immunohistochemistry (IHC) is one of the main techniques currently used in the clinics for biomarker characterization. It consists in colorimetric labeling with specific antibodies followed by microscopy analysis. The results are then used for diagnosis and therapeutic targeting. Well-known drawbacks of such protocols are their limited accuracy and precision, which prevent the clinicians from having quantitative and robust IHC results. With our work, we combined rapid microfluidic immunofluorescent staining with efficient image-based cell segmentation and signal quantification to increase the robustness of both experimental and analytical protocols. The experimental protocol is very simple and based on fast-fluidic-exchange in a microfluidic chamber created on top of the formalin-fixed-paraffin-embedded (FFPE) slide by clamping it a silicon chip with a polydimethyl siloxane (PDMS) sealing ring. The image-processing protocol is based on enhancement and subsequent thresholding of the local contrast of the obtained fluorescence image. As a case study, given that the human epidermal growth factor receptor 2 (HER2) protein is often used as a biomarker for breast cancer, we applied our method to HER2+ and HER2− cell lines. We report very fast (5 minutes) immunofluorescence staining of both HER2 and cytokeratin (a marker used to define the tumor region) on FFPE slides. The image-processing program can segment cells correctly and give a cell-based quantitative immunofluorescent signal. With this method, we found a reproducible well-defined separation for the HER2-to-cytokeratin ratio for positive and negative control samples.

Paper Details

Date Published: 19 February 2018
PDF: 6 pages
Proc. SPIE 10491, Microfluidics, BioMEMS, and Medical Microsystems XVI, 1049110 (19 February 2018); doi: 10.1117/12.2287884
Show Author Affiliations
D. Migliozzi, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
H. T. Nguyen, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
M. A. M. Gijs, Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 10491:
Microfluidics, BioMEMS, and Medical Microsystems XVI
Bonnie L. Gray; Holger Becker, Editor(s)

© SPIE. Terms of Use
Back to Top