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Hybrid adaptive and computational light-sheet fluorescence microscopy
Author(s): Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Reto Fiolka; Michel Verhaegen
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Paper Abstract

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path providing the optical sectioning and the fluorescence detection path are both affected by the aberrations in different ways. To overcome these difficulties, we have developed hybrid adaptive optical and computational microscopy techniques to remove the effect of the aberrations in both the excitation and the fluorescence paths of these microscopes.

Paper Details

Date Published: 23 February 2018
PDF: 7 pages
Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 1050212 (23 February 2018); doi: 10.1117/12.2287661
Show Author Affiliations
Dean Wilding, Technische Univ. Delft (Netherlands)
Paolo Pozzi, Technische Univ. Delft (Netherlands)
Oleg Soloviev, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
Gleb Vdovin, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
Reto Fiolka, The Univ. of Texas Southwestern (United States)
Michel Verhaegen, Technische Univ. Delft (Netherlands)


Published in SPIE Proceedings Vol. 10502:
Adaptive Optics and Wavefront Control for Biological Systems IV
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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