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Practical guidelines for implementing adaptive optics in fluorescence microscopy
Author(s): Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen
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Paper Abstract

In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.

Paper Details

Date Published: 23 February 2018
PDF: 12 pages
Proc. SPIE 10502, Adaptive Optics and Wavefront Control for Biological Systems IV, 105021F (23 February 2018); doi: 10.1117/12.2287647
Show Author Affiliations
Dean Wilding, Technische Univ. Delft (Netherlands)
Paolo Pozzi, Technische Univ. Delft (Netherlands)
Oleg Soloviev, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
ITMO Univ. (Russian Federation)
Gleb Vdovin, Technische Univ. Delft (Netherlands)
Flexible Optical B.V. (Netherlands)
ITMO Univ. (Russian Federation)
Michel Verhaegen, Technische Univ. Delft (Netherlands)


Published in SPIE Proceedings Vol. 10502:
Adaptive Optics and Wavefront Control for Biological Systems IV
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

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