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Laser-assisted decontamination of biofilm in flexible cystoscope (Conference Presentation)
Author(s): Van Nam Tran; Chakradhar Dasagrandhi; Van Gia Truong; Young Mog Kim; Hyun Wook Kang
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Paper Abstract

Cystoscope cleaning and sterilization are one of the most important procedures to prevent infection in urological surgery. The current cleaning techniques have demonstrated lengthy process times or insufficient decontamination of flexible cystoscopes. Thus, high-level disinfection and sterilization are still needed to enhance cleaning efficacy. The current study demonstrated the feasibility of a combination of 405-nm laser and infrared (IR) lights to inactivate bacterial biofilms inside the working channels. Staphylococcus strains in suspension (concentration ~ 108 CFU/mL) were incubated in 24 hours at 37 0C to form biofilms on multi-well plates. Each sample initially experienced 5-minute IR dehydration (intensity = 1.5 W/cm2), and 405-nm laser system was then used for bacterial disinfection at various conditions: 19.1, 38.2, 57.3, and 76.4 J/cm2. An IR camera was deployed for real-time monitoring temperature on the biofilm surface. MTT assay was employed to assess cell viability after the treatment. The results showed that staphylococcus bacteria in liquid suspension were more resistant to 405-nm laser light than those in hard surfaces. In addition, the 405-nm laser treatment resulted in less decontaminating effectiveness while the 405-nm laser in conjunction with IR light could induce bacterial reduction up to 75% possibly due to effect low temperature dehydration. The proposed technique can be a useful modality for cystoscope cleaning and sterilization to prevent any secondary infection in the urinary tract during urological procedures. Further experiments will be conducted to validate reprocessing efficacy on various types of bacterial biofilm models along with high-level disinfectants to find the optimal cleaning doses.

Paper Details

Date Published: 14 March 2018
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Proc. SPIE 10468, Therapeutics and Diagnostics in Urology 2018, 1046802 (14 March 2018); doi: 10.1117/12.2287644
Show Author Affiliations
Van Nam Tran, Pukyong National Univ. (Korea, Republic of)
Chakradhar Dasagrandhi, Pukyong National Univ. (Korea, Republic of)
Van Gia Truong, Pukyong National Univ. (Korea, Republic of)
Young Mog Kim, Pukyong National Univ. (Korea, Republic of)
Hyun Wook Kang, Pukyong National Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 10468:
Therapeutics and Diagnostics in Urology 2018
Hyun Wook Kang; Kin Foong Chan, Editor(s)

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