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Proceedings Paper

DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems
Author(s): Lingling Chen; Lina Liu; Guiye Li; Ang Liu; Yingchao Li; Xuejuan Hu; Shuangchen Ruan
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Paper Abstract

There is increasing interest in the three-dimensional visualization and quantification of cellular circuits in the brain and therefore optical clearing methods are highly in demand for brain imaging. In particular, clarification without membrane damage is required to image lipophilic tracer-labeled neural tracts. However, previously reported DiI-compatible optical clearing methods are relatively slow and can hinder transparency for imaging. Here, we present DAS, a new, convenient, inexpensive and reproducible aqueous clearing reagent that can efficiently clarify tissues with minimal volume enlargement and reliably preserves emission from fluorescent proteins and lipophilic dyes in membrane integrity preserved tissues.

Paper Details

Date Published: 13 February 2018
PDF: 4 pages
Proc. SPIE 10493, Dynamics and Fluctuations in Biomedical Photonics XV, 104930Y (13 February 2018); doi: 10.1117/12.2287608
Show Author Affiliations
Lingling Chen, Shenzhen Univ. (China)
Lina Liu, Shenzhen Univ. (China)
Guiye Li, Shenzhen Univ. (China)
Ang Liu, Shenzhen Univ. (China)
Yingchao Li, Shenzhen Univ. (China)
Xuejuan Hu, Shenzhen Univ. (China)
Shuangchen Ruan, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 10493:
Dynamics and Fluctuations in Biomedical Photonics XV
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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