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Fast focus-scanning head in two-photon photoacoustic microscopy with electrically-controlled liquid lens
Author(s): Yoshihisa Yamaoka; Yuka Kimura; Yoshinori Harada; Tetsuro Takamatsu; Eiji Takahashi
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Paper Abstract

Conventional one-photon photoacoustic microscopy (PAM) utilizes high-frequency components of generated photoacoustic waves to improve the depth resolution. However, to obtain optically-high resolution in PAM in the depth direction, the use of high-frequency ultrasonic waves is to be avoided. It is because that the propagation distance is shortened as the frequency of ultrasonic waves becomes high. To overcome this drawback, we have proposed and developed two-photon photoacoustic microscopy (TP-PAM). Two-photon absorption occurs only at the focus point. TPPAM does not need to use the high-frequency components of photoacoustic waves. Thus, TP-PAM can improve the penetration depth while preserving the spatial resolution. However, the image acquisition time of TP-PAM is longer than that of conventional PAM, because TP-PAM needs to scan the laser spot both in the depth and transverse directions to obtain cross-sectional images. In this paper, we have introduced a focus-tunable electrically-controlled liquid lens in TP-PAM. Instead of a mechanical stepping-motor stage, we employed electrically-controlled liquid lens so that the depth of the focus spot can be quickly changed. In our system, the imaging speed of TP-PAM using the liquid lens and one-axis stepping-motor stage was 10 times faster than that using a two-axis stepping-motor stage only. TP-PAM with focus-scanning head consisting of the liquid lens and stepping-motor stage will be a promising method to investigate the inside of living tissues.

Paper Details

Date Published: 19 February 2018
PDF: 5 pages
Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942J (19 February 2018); doi: 10.1117/12.2287391
Show Author Affiliations
Yoshihisa Yamaoka, Saga Univ. (Japan)
Yuka Kimura, Saga Univ. (Japan)
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)
Eiji Takahashi, Saga Univ. (Japan)

Published in SPIE Proceedings Vol. 10494:
Photons Plus Ultrasound: Imaging and Sensing 2018
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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