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Proceedings Paper

Hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic imaging
Author(s): Zhenyue Chen; Xosé Luís Deán-Ben; Sven Gottschalk; Daniel Razansky
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Paper Abstract

Fluorescence imaging is widely employed in all fields of cell and molecular biology due to its high sensitivity, high contrast and ease of implementation. However, the low spatial resolution and lack of depth information, especially in strongly-scattering samples, restrict its applicability for deep-tissue imaging applications. On the other hand, optoacoustic imaging is known to deliver a unique set of capabilities such as high spatial and temporal resolution in three dimensions, deep penetration and spectrally-enriched imaging contrast. Since fluorescent substances can generate contrast in both modalities, simultaneous fluorescence and optoacoustic readings can provide new capabilities for functional and molecular imaging of living organisms. Optoacoustic images can further serve as valuable anatomical references based on endogenous hemoglobin contrast. Herein, we propose a hybrid system for in vivo real-time planar fluorescence and volumetric optoacoustic tomography, both operating in reflection mode, which synergistically combines the advantages of stand-alone systems. Validation of the spatial resolution and sensitivity of the system were first carried out in tissue mimicking phantoms while in vivo imaging was further demonstrated by tracking perfusion of an optical contrast agent in a mouse brain in the hybrid imaging mode. Experimental results show that the proposed system effectively exploits the contrast mechanisms of both imaging modalities, making it especially useful for accurate monitoring of fluorescence-based signal dynamics in highly scattering samples.

Paper Details

Date Published: 19 February 2018
PDF: 7 pages
Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104946C (19 February 2018); doi: 10.1117/12.2287341
Show Author Affiliations
Zhenyue Chen, Helmholtz Zentrum München GmbH (Germany)
Xosé Luís Deán-Ben, Helmholtz Zentrum München GmbH (Germany)
Sven Gottschalk, Helmholtz Zentrum München GmbH (Germany)
Daniel Razansky, Helmholtz Zentrum München GmbH (Germany)
Technische Univ. München (Germany)


Published in SPIE Proceedings Vol. 10494:
Photons Plus Ultrasound: Imaging and Sensing 2018
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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