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Proceedings Paper

Superresolution fluorescence imaging by pump-probe setup using repetitive stimulated transition process
Author(s): Fumihiro Dake; Naoki Fukutake; Seri Hayashi; Yusuke Taki
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Paper Abstract

We proposed superresolution nonlinear fluorescence microscopy with pump-probe setup that utilizes repetitive stimulated absorption and stimulated emission caused by two-color laser beams. The resulting nonlinear fluorescence that undergoes such a repetitive stimulated transition is detectable as a signal via the lock-in technique. As the nonlinear fluorescence signal is produced by the multi-ply combination of incident beams, the optical resolution can be improved. A theoretical model of the nonlinear optical process is provided using rate equations, which offers phenomenological interpretation of nonlinear fluorescence and estimation of the signal properties. The proposed method is demonstrated as having the scalability of optical resolution. Theoretical resolution and bead image are also estimated to validate the experimental result.

Paper Details

Date Published: 20 February 2018
PDF: 6 pages
Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000Q (20 February 2018); doi: 10.1117/12.2287325
Show Author Affiliations
Fumihiro Dake, Nikon Corp. (Japan)
Naoki Fukutake, Nikon Corp. (Japan)
Seri Hayashi, Nikon Corp. (Japan)
Yusuke Taki, Nikon Corp. (Japan)

Published in SPIE Proceedings Vol. 10500:
Single Molecule Spectroscopy and Superresolution Imaging XI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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