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HeLa cells response to photodynamic treatment with Radachlorin at various irradiation parameters
Author(s): A. V. Belashov; A. A. Zhikhoreva; T. N. Belyaeva; E. S. Kornilova; N. V. Petrov; A. V. Salova; I. V. Semenova; O. S. Vasyutinskii
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Paper Abstract

Measurements of average phase shifts introduced by living HeLa cells to probe wave front were carried out. Variations of this value were monitored in the course of morphological changes caused by photodynamic treatment at various irradiation doses. Observations of changes in living cells were also performed by means of far field optical microscopy and confocal fluorescent microscopy. Quantitative analysis of the data obtained shows that average phase shift introduced by the cells may either increase or decrease depending upon major parameters of the treatment.

Paper Details

Date Published: 28 July 2017
PDF: 7 pages
Proc. SPIE 10414, Advances in Microscopic Imaging, 104140U (28 July 2017); doi: 10.1117/12.2287208
Show Author Affiliations
A. V. Belashov, Ioffe Institute (Russian Federation)
ITMO Univ. (Russian Federation)
A. A. Zhikhoreva, Ioffe Institute (Russian Federation)
ITMO Univ. (Russian Federation)
T. N. Belyaeva, Institute of Cytology (Russian Federation)
E. S. Kornilova, Institute of Cytology (Russian Federation)
N. V. Petrov, ITMO Univ. (Russian Federation)
A. V. Salova, Institute of Cytology (Russian Federation)
I. V. Semenova, Ioffe Institute (Russian Federation)
O. S. Vasyutinskii, Ioffe Institute (Russian Federation)


Published in SPIE Proceedings Vol. 10414:
Advances in Microscopic Imaging
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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