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Proceedings Paper

CARS molecular fingerprinting using a sub-nanosecond supercontinuum light source
Author(s): Hideaki Kano; Toshihiro Akiyama; Akihito Inoko; Tsubasa Kobayashi; Philippe Leproux; Vincent Couderc; Yuichi Kaji; Tetsuro Oshika
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Paper Abstract

We have visualized living cells and tissues using an ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system by using a sub-nanosecond supercontinuum (SC) light source. Owing to the ultrabroadband spectral profile of the SC, we can generate multiplex CARS signals in the spectral range of 500–3800 cm−1, which covers the whole molecular fingerprint region, as well as the C-H and O-H stretching regions. Through the combination of the ultrabroadband multiplex CARS method with second harmonic generation (SHG) and third harmonic generation (THG) processes, we have successfully performed selective imaging of ciliary rootlet-composing Rootletin filaments in rat retina.

Paper Details

Date Published: 23 February 2018
PDF: 5 pages
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981P (23 February 2018); doi: 10.1117/12.2287168
Show Author Affiliations
Hideaki Kano, Univ. of Tsukuba (Japan)
Toshihiro Akiyama, Univ. of Tsukuba (Japan)
Akihito Inoko, Aichi Cancer Ctr. Research Institute (Japan)
Tsubasa Kobayashi, Univ. of Tsukuba (Japan)
Philippe Leproux, Institut de Recherche XLIM, CNRS (France)
LEUKOS (France)
Vincent Couderc, Institut de Recherche XLIM, CNRS (France)
Yuichi Kaji, Univ. of Tsukuba (Japan)
Tetsuro Oshika, Univ. of Tsukuba (Japan)


Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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