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Proceedings Paper

Probing energy metabolism and microviscosity in cancer using FLIM
Author(s): Marina V. Shirmanova; Maria M. Lukina; Lyubov’ E. Shimolina; Marina K. Kuimova; Varvara V. Dudenkova; Vladislav I. Shcheslavskiy; Elena V. Zagaynova
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a promising non-invasive highly sensitive technique for probing multiple physiological and physicochemical parameters in living cells and tissues. The present study is focused on the investigation of bioenergetics and microscopic viscosity of cultured cancer cells and animal tumors using FLIM during natural growth and chemotherapy. Fluorescence lifetime measurements of the metabolic cofactor NAD(P)H revealed a decrease of the relative amplitude of free NAD(P)H after cisplatin treatment, indicating a change towards a more oxidative metabolic state. Microviscosity mapping performed with the use of fluorescent molecular rotor BODIPY-2 showed a pronounced increase in the plasma membrane viscosity in cancer cells exposed to cisplatin. Although biochemical mechanisms underlying the metabolic and viscosity alterations during chemotherapy have yet to be clarified, our data suggest that the cisplatin-induced changes in cellular metabolism and membrane viscosity play a role in the cytotoxicity of the drug. The results of the study contribute to an understanding of mechanisms of cisplatin action and will be useful for development new approach for assessing response to a therapy.

Paper Details

Date Published: 28 July 2017
PDF: 6 pages
Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 1041102 (28 July 2017); doi: 10.1117/12.2287094
Show Author Affiliations
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Maria M. Lukina, Nizhny Novgorod State Medical Academy (Russian Federation)
Lyubov’ E. Shimolina, Nizhny Novgorod State Medical Academy (Russian Federation)
Marina K. Kuimova, Imperial College London (United Kingdom)
Varvara V. Dudenkova, Nizhny Novgorod State Medical Academy (Russian Federation)
Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany)
Nizhny Novgorod State Medical Academy (Russian Federation)
Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)

Published in SPIE Proceedings Vol. 10411:
Clinical and Preclinical Optical Diagnostics
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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