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Proceedings Paper

A quaternary ammonium modified coumarin derivative for antimicrobial photodynamic therapy
Author(s): Zhiyuan Sun; Shaona Zhou; Ying Gu; Yuxia Zhao
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Paper Abstract

A new cationic modified coumarin derivative, 7-diethylamino-3-(3-(4-(trimethylbenzenaminium iodide) phenyl) acryloyl)-2H-chromen-2-one (1), was synthesized and characterized by 1H NMR and mass spectra. It had a strong intramolecular charge transfer absorption band around 460 nm with large molar extinction coefficients of 3.94 × 104 M-1 cm-1 in DMF and 3.86 × 104 M-1 cm-1 in PBS, respectively. Moreover, a moderate singlet oxygen quantum yield of 0.16 was obtained for 1 in DMF. Using methylene blue (MB) under a 630 nm laser as reference, the in vitro antimicrobial photodynamic therapy (aPDT) activity of 1 against three strains, gram positive bacteria methicillin-resistant staphylococcus aureus (MRSA), negative bacteria acinetobacter baumannii (A. baumannii) and fungus Candida albicans (C. albicans), was carried out by irradiation with a 457 nm laser. It was shown that 1 had no dark toxicity to these bacteria when its concentration was up to 100 μM, while under the 457 nm laser it could kill them effectively with an over 3 log CFU/ml decrease of the bacterial viability with its concentration up to 5 μM. The aPDT capability of 1 against MRSA and A. baumannii was equivalent to that of MB. For C. albicans, 1 exhibited much better aPDT effect than MB.

Paper Details

Date Published: 8 February 2018
PDF: 8 pages
Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 104790Z (8 February 2018); doi: 10.1117/12.2287086
Show Author Affiliations
Zhiyuan Sun, Technical Institute of Physics and Chemistry (China)
Univ. of Chinese Academy of Sciences (China)
Shaona Zhou, Chinese PLA General Hospital (China)
Ying Gu, Chinese PLA General Hospital (China)
Yuxia Zhao, Technical Institute of Physics and Chemistry (China)

Published in SPIE Proceedings Vol. 10479:
Light-Based Diagnosis and Treatment of Infectious Diseases
Tianhong Dai, Editor(s)

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