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Proceedings Paper

Hyper-spectrum scanning laser optical tomography
Author(s): Lingling Chen; Guiye Li; Yingchao Li; Lina Liu; Ang Liu; Xuejuan Hu; Shuangchen Ruan
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Paper Abstract

We describe a quantitative fluorescence projection tomography technique which measures the three-dimensional fluorescence spectrum in biomedical samples with size up to several millimeters. This is achieved by acquiring a series of hyperspectral images, by using laser scanning scheme, at different projection angles. We demonstrate that this technique provide a quantitative measure of the fluorescence signal by comparing the spectrum and intensity profile of a fluorescent bead phantom and also demonstrate its application to differentiating the extrinsic label and the autofluorescence in a mouse embryo.

Paper Details

Date Published: 23 February 2018
PDF: 4 pages
Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104991L (23 February 2018); doi: 10.1117/12.2287062
Show Author Affiliations
Lingling Chen, Shenzhen Univ. (China)
Guiye Li, Shenzhen Univ. (China)
Yingchao Li, Shenzhen Univ. (China)
Lina Liu, Shenzhen Univ. (China)
Ang Liu, Shenzhen Univ. (China)
Xuejuan Hu, Shenzhen Univ. (China)
Shuangchen Ruan, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 10499:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV
Thomas G. Brown; Carol J. Cogswell; Tony Wilson, Editor(s)

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