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Proceedings Paper

The microstructural variation during tissue optical clearing by Mueller matrix polarimetry
Author(s): Yu Huang; Nan Zeng; Qiaolin Xie; Valery V. Tuchin; Hui Ma
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Paper Abstract

It has been demonstrated that Mueller matrix imaging is capable of extracting the microstructures and optical properties of the tissue. We try to explain how different clearing agent affects the microstructure of the tissues during the optical clearing process using Mueller matrix analysis. We employ two type agents and record how the Mueller matrix elements vary with the immersion time, and then we compare the dynamic polarization information with the Monte Carlo simulations focusing on different possible clearing mechanisms. By analyzing the connection between Mueller matrix elements and the microstructural variation and optical features of tissues, we try to explain the microscopic phenomena induced by different agents. Then we simulated several possible cases of microstructural changes caused by tissue lesions, and then observe the influence of different agent on polarization imaging contrast by the simulation process of the possible clearing mechanisms. These research works not only confirm that Mueller matrix imaging combined with Monte Carlo simulation is potentially a powerful tool for monitoring and understanding tissue optical clearing, but also show how tissue clearing agents can improve the polarization identification and what type tissue can be improved clearer.

Paper Details

Date Published: 13 February 2018
PDF: 11 pages
Proc. SPIE 10493, Dynamics and Fluctuations in Biomedical Photonics XV, 104930X (13 February 2018); doi: 10.1117/12.2287050
Show Author Affiliations
Yu Huang, Tsinghua Univ. (China)
Nan Zeng, Tsinghua Univ. (China)
Qiaolin Xie, Tsinghua Univ. (China)
Valery V. Tuchin, Saratov State Univ. (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)
Hui Ma, Tsinghua Univ. (China)
Tsinghua-Berkeley Shenzhen Institute (China)


Published in SPIE Proceedings Vol. 10493:
Dynamics and Fluctuations in Biomedical Photonics XV
Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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