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In vivo two-photon imaging of macrophage activities in skeletal muscle regeneration
Author(s): Zhongya Qin; Yanyang Long; Qiqi Sun; Sicong He; Xuesong Li; Congping Chen; Zhenguo Wu; Jianan Y. Qu
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Paper Abstract

Macrophages are essential for the regeneration of skeletal muscle after injury. It has been demonstrated that depletion of macrophages results in delay of necrotic fiber phagocytosis and decreased size of regenerated myofibers. In this work, we developed a multi-modal two-photon microscope system for in vivo study of macrophage activities in the regenerative and fibrotic healing process of injured skeletal muscles. The system is capable to image the muscles based on the second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) signals simultaneously. The dynamic activities of macrophages and muscle satellite cells are recorded in different time windows post the muscle injury. Moreover, we found that infiltrating macrophages emitted strong autofluorescence in the injured skeletal muscle of mouse model, which has not been reported previously. The macrophage autofluorescence was characterized in both spectral and temporal domains. The information extracted from the autofluorescence signals may facilitate the understanding on the formation mechanisms and possible applications in biological research related to skeletal muscle regeneration.

Paper Details

Date Published: 20 February 2018
PDF: 6 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 1049704 (20 February 2018); doi: 10.1117/12.2286834
Show Author Affiliations
Zhongya Qin, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Yanyang Long, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Qiqi Sun, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Xuesong Li, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Congping Chen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Zhenguo Wu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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