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Labeling single cell for in-vivo study of cell fate mapping and lineage tracing
Author(s): Sicong He; Jin Xu; Yi Wu; Ye Tian; Qiqi Sun; Zilong Wen; Jianan Y. Qu
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Paper Abstract

Cell fate mapping and lineage tracing are significant ways to understanding the developmental origins of biological tissues. It requires labeling individual cells and tracing the development of their progeny. We develop an infrared laser-evoked gene operator heat-shock microscope system to achieve single-cell labeling in zebrafish. With a fluorescent thermometry technique, we measure the temperature increase in zebrafish tissues induced by infrared laser and identify the optimal heat shock conditions for single-cell gene induction in different types of zebrafish cells. We use this technique to study the fate mapping of T lymphocytes and discover the distinct waves of lymphopoiesis during the zebrafish development.

Paper Details

Date Published: 20 February 2018
PDF: 6 pages
Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970J (20 February 2018); doi: 10.1117/12.2286819
Show Author Affiliations
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jin Xu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Yi Wu, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Ye Tian, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Qiqi Sun, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Zilong Wen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 10497:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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