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Proceedings Paper

Fiber-based 1150-nm femtosecond laser source for the label-free virtual optical biopsy (Conference Presentation)
Author(s): Tzu-Ming Liu; Jing-Yu Huang; Lun-Zhang Guo; Jing-Zun Wang; Tse-Chung Li; Hsin-Jung Lee; Po-Kai Chiu; Lung-Han Peng

Paper Abstract

For deep imaging depth and least invasiveness, people commonly use 1100-1300 nm femtosecond laser sources to perform label-free in vivo microscopy. The modalities include reflectance confocal, two & three photon fluorescence, and second & third harmonic generation microscopy. However, most of the laser sources are typically based on bulky oscillators, which are sensitive to environment conditions and less stable for routine clinical use. In contrast, fiber-based lasers have simpler cavity design and potentially compact size for movable use. In this presentation, we demonstrate a fiber-based 1150 nm femtosecond laser source, with 6.5 nJ pulse energy, 86 fs pulse-width, and 11.25 MHz pulse repetition rate. It was achieved by a Bismuth Borate (BIBO) or Magnesium-doped periodically poled Lithium Niobate (MgO:PPLN) mediated frequency doubling of the 2300 nm solitons, generated from an excitation of 1550 nm Er:fiber femtosecond laser pulses on a large mode area photonic crystal fiber. Combined with a laser scanned microscope and a home-build data acquisition card, we achieve a pulse-per-pixel harmonic generation microscopy in vivo at a 30 Hz frame rate. In the future, this solution is potential to be used for label-free clinical virtual optical biopsy.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10488, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII, 1048803 (14 March 2018); doi: 10.1117/12.2286768
Show Author Affiliations
Tzu-Ming Liu, Univ. of Macau (Macao, China)
Jing-Yu Huang, National Taiwan Univ. (Taiwan)
Lun-Zhang Guo, National Taiwan Univ. (Taiwan)
Jing-Zun Wang, National Taiwan Univ. (Taiwan)
Tse-Chung Li, National Taiwan Univ. (Taiwan)
Hsin-Jung Lee, National Taiwan Univ. (Taiwan)
Po-Kai Chiu, National Applied Research Labs. (Taiwan)
Lung-Han Peng, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10488:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII
Israel Gannot, Editor(s)

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