Share Email Print
cover

Proceedings Paper

Integrating 4-d light-sheet imaging with interactive virtual reality to recapitulate developmental cardiac mechanics and physiology
Author(s): Yichen Ding; Jing Yu; Arash Abiri; Parinaz Abiri; Juhyun Lee; Chih-Chiang Chang; Kyung In Baek; René R. Sevag Packard; Tzung K. Hsiai
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

There currently is a limited ability to interactively study developmental cardiac mechanics and physiology. We therefore combined light-sheet fluorescence microscopy (LSFM) with virtual reality (VR) to provide a hybrid platform for 3- dimensional (3-D) architecture and time-dependent cardiac contractile function characterization. By taking advantage of the rapid acquisition, high axial resolution, low phototoxicity, and high fidelity in 3-D and 4-D (3-D spatial + 1-D time or spectra), this VR-LSFM hybrid methodology enables interactive visualization and quantification otherwise not available by conventional methods such as routine optical microscopes. We hereby demonstrate multi-scale applicability of VR-LSFM to 1) interrogate skin fibroblasts interacting with a hyaluronic acid-based hydrogel, 2) navigate through the endocardial trabecular network during zebrafish development, and 3) localize gene therapy-mediated potassium channel expression in adult murine hearts. We further combined our batch intensity normalized segmentation (BINS) algorithm with deformable image registration (DIR) to interface a VR environment for the analysis of cardiac contraction. Thus, the VR-LSFM hybrid platform demonstrates an efficient and robust framework for creating a user-directed microenvironment in which we uncovered developmental cardiac mechanics and physiology with high spatiotemporal resolution.

Paper Details

Date Published: 12 February 2018
PDF: 8 pages
Proc. SPIE 10484, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI, 104841C (12 February 2018); doi: 10.1117/12.2286296
Show Author Affiliations
Yichen Ding, Univ. of California, Los Angeles (United States)
Jing Yu, Zhejiang Univ. of Technology (China)
Arash Abiri, Univ. of California, Los Angeles (United States)
Univ. of California, Irvine (United States)
Parinaz Abiri, Univ. of California, Los Angeles (United States)
Juhyun Lee, Univ. of California, Los Angeles (United States)
Chih-Chiang Chang, Univ. of California, Los Angeles (United States)
Kyung In Baek, Univ. of California, Los Angeles (United States)
René R. Sevag Packard, Univ. of California, Los Angeles (United States)
Tzung K. Hsiai, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 10484:
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

© SPIE. Terms of Use
Back to Top