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Dual-phase stimulated Raman scattering microscopy for simultaneous two-color imaging (Conference Presentation)
Author(s): Minbiao Ji
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Paper Abstract

Two-color Stimulated Raman scattering (SRS) microscopy has shown great potential in label-free digital histology with diagnostic results similar to H&E stain. However, achieving real-time two-color SRS imaging is challenging. We have precisely engineered the pulse profiles of the Stokes beams, and fully utilized the in-phase (X) and quadrature (Y) outputs of a phase sensitive lock-in amplifier to realize simultaneous two-color SRS imaging. We have demonstrated its robustness and advantages in rapid histology, as well as real-time in vivo imaging of live animals, both in transmission and epi modes. Moreover, we have also adapted this method to other pump-probe based microscopes, such as transient absorption (TA) microscopy.

Paper Details

Date Published: 14 March 2018
Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104981N (14 March 2018); doi: 10.1117/12.2286211
Show Author Affiliations
Minbiao Ji, Fudan Univ. (China)

Published in SPIE Proceedings Vol. 10498:
Multiphoton Microscopy in the Biomedical Sciences XVIII
Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie, Editor(s)

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