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Anti-microbial peptide facilitated cytosolic delivery of metallic gold nanomaterials
Author(s): Anshika Kapur; Wentao Wang; Juan Diaz Hernandez; Scott Medina; Joel P. Schneider; Hedi Mattoussi
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Paper Abstract

The unique photophysical properties of gold nanomaterials combined with progress in developing effective surfacefunctionalization strategies has motivated researchers to employ them as tools for use in biomedical imaging, biosensing, diagnostics, photothermal therapy, and as drug and gene delivery vehicles. However, a major challenge limiting these advancements has been the unavailability of effective strategies to deliver these and other nanocrystals into the cytoplasm of live cells. In this study, we demonstrate that the use of a chemically-synthesized anti-microbial peptide, SVS-1, can promote non-endocytic uptake of both small size gold nanoparticles (AuNPs) and larger size gold nanorods (AuNRs) into mammalian cells. For this, colloidally stable AuNP and AuNRs, surface ligated with an amine-functionalized polymer, His-PIMA-PEG-OCH3/NH2 were prepared. The amine groups allow dual, covalent attachment of cysteine terminated SVS-1 (via a thioether linkage) and NHS-ester-Texas-Red dye onto the nanocrystal surfaces. We use fluorescence microscopy to demonstrate nanocrystal staining throughout the cytoplasmic volume of the cells incubated with these conjugates. More importantly, we have conducted additional endocytosis inhibition experiments where cells were incubated with the conjugates at 4°C. Here too, the imaging data have shown significant levels of nanocrystal uptake, further verifying that physical translocation of these conjugates takes place through the cell membrane independent of endocytosis. These findings are promising and can provide critical support for the widespread applications of nanomaterials in the field of biology.

Paper Details

Date Published: 23 February 2018
PDF: 8 pages
Proc. SPIE 10507, Colloidal Nanoparticles for Biomedical Applications XIII, 105070R (23 February 2018); doi: 10.1117/12.2285661
Show Author Affiliations
Anshika Kapur, Florida State Univ. (United States)
Wentao Wang, Florida State Univ. (United States)
Juan Diaz Hernandez, Florida State Univ. (United States)
Scott Medina, The Pennsylvania State Univ. (United States)
Joel P. Schneider, National Cancer Institute (United States)
Hedi Mattoussi, Florida State Univ. (United States)


Published in SPIE Proceedings Vol. 10507:
Colloidal Nanoparticles for Biomedical Applications XIII
Marek Osiński; Wolfgang J. Parak; Xing-Jie Liang, Editor(s)

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