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Proceedings Paper

Multi-spectral endogenous fluorescence imaging for bacterial differentiation
Author(s): Nikita V. Chernomyrdin; Margarita V. Babayants; Oleg V. Korotkov; Konstantin G. Kudrin; Elena N. Rimskaya; Irina A. Shikunova; Vladimir N. Kurlov; Olga P. Cherkasova; Gennady A. Komandin; Igor V. Reshetov; Kirill I. Zaytsev
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Paper Abstract

In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.

Paper Details

Date Published: 28 July 2017
PDF: 7 pages
Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110B (28 July 2017); doi: 10.1117/12.2285065
Show Author Affiliations
Nikita V. Chernomyrdin, Bauman Moscow State Technical Univ. (Russian Federation)
Margarita V. Babayants, State Humanitarian Technology Univ. (Russian Federation)
Oleg V. Korotkov, State Humanitarian Technology Univ. (Russian Federation)
Konstantin G. Kudrin, I.M. Sechenov First Moscow State Medical Univ. (Russian Federation)
Elena N. Rimskaya, I.M. Sechenov First Moscow State Medical Univ. (Russian Federation)
Irina A. Shikunova, Institute of Solid State Physics (Russian Federation)
Vladimir N. Kurlov, Institute of Solid State Physics (Russian Federation)
Olga P. Cherkasova, Institute of Laser Physics (Russian Federation)
Gennady A. Komandin, A.M. Prokhorov General Physics Institute (Russian Federation)
Igor V. Reshetov, I.M. Sechenov First Moscow State Medical Univ. (Russian Federation)
Kirill I. Zaytsev, Bauman Moscow State Technical Univ. (Russian Federation)
I.M. Sechenov First Moscow State Medical Univ. (Russian Federation)
A.M. Prokhorov General Physics Institute (Russian Federation)


Published in SPIE Proceedings Vol. 10411:
Clinical and Preclinical Optical Diagnostics
J. Quincy Brown; Ton G. van Leeuwen, Editor(s)

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