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Proceedings Paper

Research of the absorbance detection and fluorescence detection for multifunctional nutrition analyzer
Author(s): Zhengyuan Ni; Huimin Yan; Xuxiang Ni; Xiuda Zhang
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Paper Abstract

The research of the multifunctional analyzer which integrates absorbance detection, fluorescence detection, time-resolved fluorescence detection, biochemical luminescence detection methods, can make efficient detection and analysis for a variety of human body nutrients. This article focuses on the absorbance detection and fluorescence detection system. The two systems are modular in design and controlled by embedded system, to achieve automatic measurement according to user settings. In the optical path design, the application of confocal design can improve the optical signal acquisition capability, and reduce the interference. A photon counter is used for detection, and a high performance counter module is designed to measure the output of photon counter. In the experiment, we use neutral density filters and potassium dichromate solution to test the absorbance detection system, and use fluorescein isothiocyanate(FITC)for fluorescence detection system performance test. The experimental results show that the absorbance detection system has a detection range of 0~4OD, and has good linearity in the detection range, while the fluorescence detection system has a high sensitivity of 1pmol/L concentration.

Paper Details

Date Published: 24 October 2017
PDF: 6 pages
Proc. SPIE 10461, AOPC 2017: Optical Spectroscopy and Imaging, 1046110 (24 October 2017); doi: 10.1117/12.2285019
Show Author Affiliations
Zhengyuan Ni, Zhejiang Univ. (China)
Huimin Yan, Zhejiang Univ. (China)
Xuxiang Ni, Zhejiang Univ. (China)
Xiuda Zhang, Zhejiang Univ. (China)

Published in SPIE Proceedings Vol. 10461:
AOPC 2017: Optical Spectroscopy and Imaging
Jin Yu; Zhe Wang; Wei Hang; Bing Zhao; Xiandeng Hou; Mengxia Xie; Tsutomu Shimura, Editor(s)

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