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Proceedings Paper

Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction
Author(s): Irene Costantini; Miriam Menzel; Ludovico Silvestri; Nicole Schubert; Markus Axer; Katrin Amunts; Francesco S. Pavone
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Paper Abstract

In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species.

Paper Details

Date Published: 28 July 2017
PDF: 3 pages
Proc. SPIE 10413, Novel Biophotonics Techniques and Applications IV, 1041306 (28 July 2017); doi: 10.1117/12.2284328
Show Author Affiliations
Irene Costantini, LENS - Lab. Europeo di Spettroscopie Non-Lineari, Univ. degli Studi di Firenze (Italy)
Miriam Menzel, Forschungszentrum Jülich GmbH (Germany)
Ludovico Silvestri, LENS - Lab. Europeo di Spettroscopie Non-Lineari, Univ. degli Studi di Firenze (Italy)
Nicole Schubert, Forschungszentrum Jülich GmbH (Germany)
Markus Axer, Forschungszentrum Jülich GmbH (Germany)
Katrin Amunts, Forschungszentrum Jülich GmbH (Germany)
Heinrich Heine Univ. (Germany)
Francesco S. Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari, Univ. degli Studi di Firenze (Italy)
Istituto Nazionale di Ottica, CNR (Italy)


Published in SPIE Proceedings Vol. 10413:
Novel Biophotonics Techniques and Applications IV
Arjen Amelink, Editor(s)

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