Share Email Print

Proceedings Paper

Analysis of cytoplasmic diffusion with multiphoton microscopy
Author(s): Eric J. Jervis
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Fluorescence Photobleaching Recovery (FPR) has proven to be a versatile technique for assessing the transport phenomena of fluorescently tagged proteins. Effective diffusion coefficients are estimated by observing the rate of fluorescence recovery resulting from the influx of fluorophores into a volume rendered non-fluorescent by photobleaching. Diffusion coefficients may be determined through non-linear regression of a theoretical model' or by relating half times of recovery to calibration data2. Due to the lack of axial confinement inherent in confocal FPR, analyses are limited to two-dimensional systems. However, with the development of multiphoton microscopy, three-dimensional diffusion coefficients can now be determined with a spatial resolution of a few microns3.

Paper Details

Date Published: 29 August 2017
PDF: 2 pages
Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 1031337 (29 August 2017); doi: 10.1117/12.2283911
Show Author Affiliations
Eric J. Jervis, Univ. of Waterloo (Canada)

Published in SPIE Proceedings Vol. 10313:
Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging
John C. Armitage, Editor(s)

© SPIE. Terms of Use
Back to Top