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Analysis of cytoplasmic diffusion with multiphoton microscopy
Author(s): Eric J. Jervis
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Paper Abstract

Fluorescence Photobleaching Recovery (FPR) has proven to be a versatile technique for assessing the transport phenomena of fluorescently tagged proteins. Effective diffusion coefficients are estimated by observing the rate of fluorescence recovery resulting from the influx of fluorophores into a volume rendered non-fluorescent by photobleaching. Diffusion coefficients may be determined through non-linear regression of a theoretical model' or by relating half times of recovery to calibration data2. Due to the lack of axial confinement inherent in confocal FPR, analyses are limited to two-dimensional systems. However, with the development of multiphoton microscopy, three-dimensional diffusion coefficients can now be determined with a spatial resolution of a few microns3.

Paper Details

Date Published: 29 August 2017
PDF: 2 pages
Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 1031337 (29 August 2017); doi: 10.1117/12.2283911
Show Author Affiliations
Eric J. Jervis, Univ. of Waterloo (Canada)


Published in SPIE Proceedings Vol. 10313:
Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging
John C. Armitage, Editor(s)

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