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Proceedings Paper

Two-photon photodynamic therapy: photobleaching rates
Author(s): Rebecca Goyan
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Paper Abstract

A promising development in photodynamic therapy (PDT) is the use of two-photon excitation (TPE). The confinement of the excitation volume leads to the possibility of subcellular PDT. Thus, in order to design a treatment protocol, one must be aware of where a photosensitizer localizes in a cell and the individual differences, both between cellular localization sites and individual cells. One way to determine the subcellular location is to observe photobleaching dynamics in cells and compare the rate constants to those observed in solvents which have been chosen to model different characteristics of environments, such as polarity. We have observed the photobleaching behaviour of Verteporfm (VP) in a variety of solvents and single cells and have been able to correlate subcellular position with environmental polarity.

Paper Details

Date Published: 29 August 2017
PDF: 3 pages
Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 1031330 (29 August 2017); doi: 10.1117/12.2283904
Show Author Affiliations
Rebecca Goyan, Royal Military College of Canada (Canada)

Published in SPIE Proceedings Vol. 10313:
Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging
John C. Armitage, Editor(s)

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